超声速燃烧火焰放热区结构CH-PLIF成像技术

超燃冲压发动机是吸气式高超声速飞行器的关键部件之一，超燃冲压发动机燃烧室内火焰结构的研究对揭示超声速燃烧的稳焰机理具有重要意义。利用平面激光诱导荧光（Planar Laser-Induced Fluorescence，PLIF）技术测量了超声速燃烧直连式试验台燃烧过程中重要自由基CH的二维分布，实现了超声速燃烧火焰放热区结构的可视化。在开敞空间的低速射流火焰炉中使用甲烷/空气预混火焰对CH-PLIF技术进行了初步验证和系统优化，再利用CH-PLIF技术在凹腔稳焰的超燃直连台上实现了超声速燃烧火焰放热区结构的二维可视化，并与OH-PLIF和CH自发辐射测量结果进行了对比。实验结果表明，在开敞空间的低速射流预混火焰中，火焰放热区会发生扭曲、褶皱和分裂等现象，随着雷诺数的增大，火焰锋面褶皱程度更加显著；在凹腔稳焰的超声速燃烧中，火焰放热区高度褶皱和破碎，放热区结构的厚度为0.5～6.5 mm，同时也存在放热区的分裂与剥离等现象。CH-PLIF技术能够以较高的空间分辨率更准确地呈现凹腔超声速火焰放热区的结构，其在凹腔稳焰的超声速燃烧诊断中具有重要的应用价值。     

超燃冲压发动机燃烧室燃料喷注及其内流场的数值模拟

基于三维N-S方程,利用有限差分数值离散方法,对考虑了燃料喷注和凹腔结构综合影响的某超燃冲压发动机燃烧室模型的内流场进行了数值模拟,与实验流动图谱进行了定性比较,并进一步探讨了燃料喷注过程的非定常特性以及凹腔结构对流动的影响。研究表明:本文数值模拟的稳态时刻流场与实验流动图谱相似;数值结果捕捉到了横向喷注燃料沿下游运动并向凹腔扩散的非定常动态变化的规律。     

Novel aluminum-based fuel: Facile preparation to improve thermal reactions

To improve the thermal properties of aluminum (Al) in the energetic system, a coated structure with ammonium perchlorate (AP) was prepared by a facile approach. And N, N-Dimethylformamide (DMF) was chosen as an ideal solvent based on heterogeneous nucleation theory and molecular dynamics simulation. This coated structure could enlarge the contact area and improve the reaction environment to enhance the thermal properties. The addition of AP could accelerate oxidation temperature of Al with around 17.5 °C. And the heat release of 85@15 composition rises to 26.13 kJ/g and the reaction degree is 97.6% with higher peak pressure (254.6 kPa) and rise rate (1.397 MPa/s). An ideal ratio with 15 wt% AP was probed primarily. The high energy laser-induced shockwave experiment was utilized to simulate the reaction behavior in hot field. And the larger activated mixture of coated powder could release more energy to promote the growth of shockwave with higher speed up to 518.7 ± 55.9 m/s. In conclusion, 85@15 composition is expected to be applied in energetic system as a novel metal fuel.     

应用荧光法分析汽油燃烧残留物

通过应用三维荧光光谱法,对比分析了汽油、煤油、柴油及其燃烧残留物的三维荧光特征参数,主要分析了荧光峰位置、半峰宽、荧光发光区域分布、次峰与主峰的荧光强度比等,验证了荧光光谱法在鉴别火场汽油助燃剂燃烧残留物中的可行性,为火灾原因调查提供更多依据。     

轮状病毒荧光定量PCR标准品的构建

采用MA104细胞系培养和T载体克隆技术,在轮状病毒结构蛋白VP4基因序列中设计合成引物,经过PCR扩增后将特异性产物与pMD-19-T载体连接,对获得的轮状病毒重组质粒标准品进行酶切鉴定和测序分析。利用常规PCR和荧光定量PCR方法对获得的重组质粒标准品进行特异性和灵敏度指标的检验。结果表明,将构建的重组质粒作为标准品制备标准曲线具有较高的扩增效率和良好的线性关系(斜率为-4.015,R2=0.991);荧光定量PCR熔解曲线分析表明,85.8℃的PCR产物是轮状病毒VP4基因序列的特异性产物,该标准品具有轮状病毒特异性;同时,所制备的重组质粒标准品在荧光定量PCR检测中具有较大的线性范围(5×101~5×1010拷贝/μL),每个梯度PCR反应最低可以检测到50拷贝的重组质粒,因而采用该标准品进行荧光定量PCR分析具有较高的检测灵敏度;此外,该重组质粒标准品具有较高的稳定性和重复性,3次独立实验的变异因数Cv<1.5%。构建的重组质粒标准品可以用做环境水体中轮状病毒的荧光定量PCR标准品。     

用LIIF法测量DF/HF化学激光器喷管混合性能的数值分析

激光诱导碘荧光(LIIF)是一种很好的流场测量技术,为了验证该方法测量化学激光器流场混合性能的精度,模拟得到连续波DF HF化学激光器的HYLTE喷管冷流场,并计算了用氦碘混合气体作为氧化剂流、用氦气作为燃料流的HYLTE喷管的流场。通过比较前一流场中的氟原子和后一流场中的碘分子的摩尔百分比分布情况得知,当作为氧化剂流的氦碘混合物中碘的质量百分比在54%左右时,用LIIF法测量DF HF化学激光器喷管的混合情况具有较高的精度。     

荧光分析技术在单个生物分子行为及实时检测中的应用

活细胞单分子行为及实时检测研究是生命科学向微观世界深入探索的一个重要标志，它需要生物、化学、物理等多学科的交叉，已经成为目前研究的一个热点。该领域主要研究内容有细胞内单个大分子的实时研究、细胞内大分子的超微量实时检测。文章介绍了近年来对活细胞单分子行为及实时检测研究的几种荧光分析技术及其应用，涉及到的荧光分析技术有全内反射荧光显微术、荧光共振能量转移术、多光子荧光成像术、荧光相关光谱、光漂白后荧光恢复技术、荧光寿命成像显微术、单分子荧光偏振等。这些荧光分析技术以其快速、无损、时空分辨率高的特点，成为了研究单个生物分子行为的重要手段。     

NO分子A~2Σ←X~2Π跃迁的荧光激发

利用激光光谱技术得到NO分子在 2 1 0～ 2 36nm范围内的荧光发射谱 ,谱线峰值归属于A2 Σ←X2 Π(0 ,0 )、(0 ,1 )跃迁 ,根据谱线峰值位置 ,得到A2 Σ态的基振动频率和平衡位置的力常数。     

Hyperspectral imaging and remote trace detection of cis-1,3,4,6-tetranitrooctahydroimidazo-[4,5 d] imidazole (BCHMX) compared with traditional explosives using laser induced fluorescence

cis-1,3,4,6-Tetranitrooctahydroimidazo-[4,5 d] imidazole (BCHMX) is an advanced energetic compound that expected to spread worldwide in the near future. Since, no approved remote detection methods were reported in current literature for this material, we performed hyper-spectral imaging and laser induced fluorescence (LIF) to a BCHMX sample under low laser fluence for determining the optimum laser wavelength used in any future BCHMX-LIF based remote detection systems. For this purpose, an experimental setup consisted of a sun spectrum lamp and hyper-spectral camera was built to illuminate and image white powder samples of BCHMX in comparison with the traditional explosives, HMX (1,3,5,7-tetranitro-1,3,5,7-tetraazacyclooctane), RDX (1,3,5-trinitro-1,3,5-triazacyclohexane), PETN (2,2-Bis[(nitroxy)methyl]propane-1,3-diyldinitrate). The imaging reveals strong BCHMX sample absorption contrast among other samples at wavelength ranging from 400 to 410 nm. When light source was replaced by a 405 nm laser diode illuminator, a strong BCHMX sample LIF at the spectral range from 425 to 700 nm was observed under low laser fluence condition of 0.1 mJ/cm². Finally, we demonstrated successfully the ability of the 405 nm LIF and the hyperspectral imaging technique to detect finger print traces of BCHMX on white cellulose fabric from a distance of 15 m and a detection limit of 1 μg/cm².