| 东乡野生稻NBS-LRR类抗性基因同源序列的分离与鉴定 |
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| 引用本文： | 王正华,杲修杰,曹孟良.东乡野生稻NBS-LRR类抗性基因同源序列的分离与鉴定[J].国防科技大学学报,2008,30(2):56-60. |
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| 作者姓名： | 王正华 杲修杰 曹孟良 |
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| 作者单位： | 1. 国防科技大学,计算机学院,湖南,长沙,410073
2. 国家杂交水稻工程技术研究中心,湖南,长沙,410125 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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中国博士后科学基金
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湖南省级重点实验室科研项目 |
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| 摘 要： | 基于植物的抗性基因具有保守序列,根据已克隆基因的保守序列设计探针,利用菌落杂交的方法,对东乡野生稻大片段基因组文库进行筛选,得到两个候选克隆.序列比对发现其中含有NB-ARC的保守序列,可用于后续转基因鉴定.
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| 关 键 词： | NBS-LRR 抗性基因 菌落杂交 基因组文库 |
| 文章编号： | 1001-2486(2008)02-0056-05 |
| 收稿时间： | 2007/10/14 0:00:00 |
| 修稿时间： | 2007年10月14 |
Isolation and Characterization of NBS-LRR Resistance Gene Homology Sequences from Dongxiang Wild Rice |
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| WANG Zhenghu,GAO Xiujie and CAO Mengliang.Isolation and Characterization of NBS-LRR Resistance Gene Homology Sequences from Dongxiang Wild Rice[J].Journal of National University of Defense Technology,2008,30(2):56-60. |
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| Authors: | WANG Zhenghu GAO Xiujie and CAO Mengliang |
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| Institution: | WANG Zheng-hua1,GAO Xiu-jie1,CAO Meng-liang2(1.College of Computer,National Univ.of Defense Technology,Changsha 410073,China,2.National Hybrid Rice R&D Center,Changsha 410125,China) |
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| Abstract: | The resistant gene of plants has conservative sequence.Probes were designed in terms of the sequences of known resistance gene.With the colony hybridization method,the genomic library of Dongxiang wild rice was screened and two candidate clones were obtained.The sequences were analyzed with the blast program to the GenBank database and a conservative NB-ARC domain was found in both of the sequences.The clones would be transferred into rice for a further analysis. |
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| Keywords: | NBS-LRR resistance gene colony hybridization genomic library |
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