| 京100850,北京100850,北京100850PTE |
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| 摘 要： | 、中性单细胞凝胶电泳技术,分别比较Pten / MEFs与Pten-/-MEFs细胞中ROS、8-羟基脱氧鸟苷(8-OH-dG)水平、DNA双链断裂(DSBs)以及抗氧化能力的差异。结果:Pten-/-MEFs细胞中ROS、8-OH-dG水平明显增高,DSBs增加,抗氧化能力减弱。结论:PTEN可能通过调控细胞内ROS水平拮抗DNA氧化损伤。PTEN Deletion Leads to Increased Oxidative DNA Damage in Mouse Embryonic FibroblastsGOU Qiao
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| 关 键 词： | 化学/荧光发光分析、免疫细胞化学 |
1,2 HUO Yan-ying1,HU Ying-chun1,LI Gang1,WU De-chang1(1.Beijing |
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| Abstract: | rsity of Medical Science,Chongqing 400016,China)BACKGROUND AND AIM:To study the variation of the level of reactive oxygen species(ROS),oxidative DNA damage and antioxidative ability between Pten / MEFs and Pten-/-MEFs cells.MATERIALS AND METHODS:Fluorescence activated cell sorter(FACS),immunocytochemistry(ICC)and neutral single cell gel electrophoresis(SCGE)were employed to compare the differences of the levels of ROS,8-hydroxy-2'-deoxyguanosine(8-OH-dG),double-strand breaks(DSBs)and antioxidative ability between Pten / MEFs and Pten-/-MEFs cells.RESULTS:In Pten-/-MEFs,the levels of ROS,8-OH-dG and DSBs were increased.Moreover,its antioxidative ability was d |
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| Keywords: | ecreased CONCLUSION:PTEN could regulate the level |
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