亲合层析SELEX法筛选相思子毒素寡核苷酸适配子

报道了采用亲和树脂法对相思子毒素蛋白进行反复的SELEX筛选,获得与靶物质结合率这32．5％的富集文库,并克隆得到了单克隆适配子序列,软件模拟了这些序列的二级结构,选择部分序列选择性剪切后利用荧光偏振方法进行检测,对靶蛋白的检测限达到亚nmol量。     

Blast resistance evaluation of urban utility tunnel reinforced with BFRP bars

To improve corrosion-resistance of shallow-buried concrete urban utility tunnels(UUTs),basalt fiber reinforced polymer(BFRP)bars are applied to reinforce UUTs.As the UUT must have excellent survival capability under accidental explosions,a shallow-buried BFRP bars reinforced UUT(BBRU)was designed and constructed.Repetitive blast experiments were carried out on this BBRU.Dynamic responses,damage evolutions and failure styles of the BBRU under repetitive explosions were revealed.The tunnel roof is the most vulnerable component and longitudinal cracks develop along the tunnel.When the scaled distance is larger than 1.10 m/kg1/3,no cracks are observed in the experiments.When the BBRU is severely damaged,there are five cracks forming and developing along the roof.The roof is simplified as a clamped-supported one-way slab,proved by the observation that the maximum strain of the transverse bar is much larger than that of the longitudinal bar.Dynamic responses of the roof slab are predicted by dynamic Euler beam theory,which can consistently predict the roof displacement under large-scaled-distance explosion.Compared with the UUT reinforced with steel bars,the BBRU has advantages in blast resistance with smaller deflections and more evenly-distributed cracks when the scaled distance is smaller than 1.260 m/kg1/3 and the steel bars enter plastic state.Longer elastic defamation of the BFRP bars endows the UUT more excellent blast resistance under small-scaled-distance explosions.     

Statistical equivalency and optimality of simple step‐stress accelerated test plans for the exponential distribution

Accelerated life testing (ALT) is commonly used to obtain reliability information about a product in a timely manner. Several stress loading designs have been proposed and recent research interests have emerged concerning the development of equivalent ALT plans. Step‐stress ALT (SSALT) is one of the most commonly used stress loadings because it usually shortens the test duration and reduces the number of required test units. This article considers two fundamental questions when designing a SSALT and provides formal proofs in answer to each. Namely: (1) can a simple SSALT be designed so that it is equivalent to other stress loading designs? (2) when optimizing a multilevel SSALT, does it degenerate to a simple SSALT plan? The answers to both queries, under certain reasonable model assumptions, are shown to be a qualified YES. In addition, we provide an argument to support the rationale of a common practice in designing a SSALT, that is, setting the higher stress level as high as possible in a SSALT plan. © 2012 Wiley Periodicals, Inc. Naval Research Logistics, 2013     

试论20世纪20年代中国留苏军事教育与中国革命

20世纪20年代中国的留苏教育不仅为中国培养了革命干部,而且为中国共产党造就了一批军事人才。以往人们对前者关注较多,而对后者注意甚少。本文试图探讨20世纪20年代留苏军事教育的发展过程及其与中国国内革命形势的关系和对后来中国革命的影响。     

神经网络距离-多普勒超分辨雷达成像

研究利用神经网络实现超分辨雷达成像。该方法首先根据Tuff的前后向线性预测(FBLP)方程用神经网络得到样本序列的AR模型参数,然后利用神经网络作线性预测外推,再用神经网络对外推后的数据作离散哈特来变换(DHT)。从哈特来变换可以方便地得到外推数据的离散傅里叶变换,从而得到距离-多普勒超分辨雷达像。利用微波暗室数据对这一方法进行了数字仿真,结果表明这种方法是可行的,即使在低信噪比下也有较好的性能。     

构件和运动副的工作能力的定量描述

继建立起机构工作能力的计算模型之后,讨论如何将构件和运动副的工作能力定量化,并给出了计算示例,从而使机构工作能力的定量分析更为明了.     

高频湖底混响具有混沌性的探讨

用５种不同底质（淤泥、岩石、卵石、沙和沙砾）的湖底混响数据探讨了高频混响具有混沌特性．该结论将有助于提高混响背景下信号检测性能．