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| 浓缩富集＋基因芯片快速检测水体中致病菌 | |
| 引用本文： | 王大勇,肖进文,方振东,王昱,敖漉,张春秀.浓缩富集＋基因芯片快速检测水体中致病菌[J].后勤工程学院学报,2010,26(4):65-70,91. |
| 作者姓名： | 王大勇 肖进文 方振东 王昱 敖漉 张春秀 |
| 作者单位： | 1. 后勤工程学院,营房管理与环境工程系,重庆,401311 2. 重庆进出口食品安全检测技术工程中心,重庆,400020 3. 后勤工程学院,重庆,401311 4. 重庆进出口食品安全检测技术工程中心,重庆,400020;重庆大学,重庆,400030 5. 上海生物芯片有限公司,上海,201203;生物芯片上海国家工程研究中心,上海,201203 |
| 基金项目： | 重庆市科技攻关计划资助项目 |
| 摘 要： | 为了实现对水体致病菌的快速检测和监控，研究开发了一套预浓缩富集配合基因芯片检测的方法，对模拟和实际水样进行了检测，并与传统检测方法进行了比较。结果表明，通过对水样的浓缩集菌，基因芯片对致病菌的检出率（以肠炎沙门氏菌为单一检测对象）达到1．0×10^3cfu／mL，较原来提高了100倍；可以在8h内同步完成13种对人类有重大危害的致病菌的快速检测。 |
| 关 键 词： | 水体致病菌 浓缩富集 基因芯片检测 |
Pre-concentration+Gene Chip Detection Method Used for the Detection of Pathogens in Water Body |
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| WANG Da-yong,XIAO Jin-wen,FANG Zhen-dong,WANG Yu,AO Lu,ZHANG Chun-xiu.Pre-concentration+Gene Chip Detection Method Used for the Detection of Pathogens in Water Body[J].Journal of Logistical Engineering University,2010,26(4):65-70,91. | |
| Authors: | WANG Da-yong XIAO Jin-wen FANG Zhen-dong WANG Yu AO Lu ZHANG Chun-xiu |
| Institution: | 1. Dept. of Barracks＇ Management ＆ Environmental Engineering, LEU, Chongqing 401311, China ; 2. Chongqing Engineering Research Center for Import and Export Food Safety, Chongqing 400020, China ;3. Logistical Engineering University, Chongqing 401311, China ;4. Chongqing University, Chongqing 400030, China ;5. Shanghai Biochip Co. Ltd, Shanghai 201203, China; 6. National Engineering Center for Bioehip at Shanghai, Shanghai 201203, China) |
| Abstract: | Abstract In this study, a rapid pre-concentration ＋ gene chip detection method is developed for the detection and monitor pathogenic microorganism in natural water. After enrichment of pathogens by the Milliflex Plus concentrator,the detection rate of pure culture bacteria is 1.0×10^3 cfu/mL,which increases 100 times. At the same time,the detection duration reduces to 8 h. AS is observed in the research, the pre-concentration and gene mieroarray detection method can be one prosperous screen method for the detection of pathogenic microorganism pollution in the natural water body. |
| Keywords: | pathogens in water pre-concentration gene chip detection |
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