| 直接免疫PCR方法的建立 |
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| 引用本文： | 宋云扬 刘娟 应天翼 张靖 郝兰群 李艳军 王惠芳. 直接免疫PCR方法的建立[J]. 防化研究, 2005, 0(4): 4-7 |
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| 作者姓名： | 宋云扬 刘娟 应天翼 张靖 郝兰群 李艳军 王惠芳 |
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| 摘 要： | 免疫PCR基本原理与ELISA相似，即用一段可扩增的DNA分子代替ELISA中酶放大检出信号,建立了直接免疫PCR的检测蓖麻毒素技术，实验结果表明，其灵敏度达1pg／ml，比ELISA方法灵敏度高10^3倍.
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| 关 键 词： | 蓖麻毒素 免疫PCR 戊二醛 生物素 亲和素 |
| 收稿时间： | 2005-02-24 |
Establishment of direct immuno-PCR technique for detection of ricin |
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| Song Yunyang, Liu Juan, Ying Tianyi, Hao Lanqun, Zhang Jing, Li Yanjun, Wan Huifang. Establishment of direct immuno-PCR technique for detection of ricin[J]. , 2005, 0(4): 4-7 |
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| Authors: | Song Yunyang Liu Juan Ying Tianyi Hao Lanqun Zhang Jing Li Yanjun Wan Huifang |
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| Abstract: | Immuno-PCR is basically similar to the enzyme-linked immunosobent assay which detects an antigen-antibody reaction, but instead of using an enzyme such as peroxidase or alkalinephosphatase conjugated to an antibody, a DNA fragment is used and this DNA can be amplified by probe. Ricin is detected by means of a newly established method, termed immuno-PCR. Result shows that as little as 1 pg/ml of ricin can be detected by the technique, which is 10^3 times more sensitive than ELISE parallel control. |
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| Keywords: | ricin immuno-PCR glutaraldehyde biotin avidin |
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