亲合层析SELEX法筛选相思子毒素寡核苷酸适配子

报道了采用亲和树脂法对相思子毒素蛋白进行反复的SELEX筛选,获得与靶物质结合率这32．5％的富集文库,并克隆得到了单克隆适配子序列,软件模拟了这些序列的二级结构,选择部分序列选择性剪切后利用荧光偏振方法进行检测,对靶蛋白的检测限达到亚nmol量。     

略论思想政治工作中的形象思维

在思想政治工作实践中 ,作为重要的非理性思维因素在工作主体的思维方式中是大量存在的 ,并且可以“配合”理性思维 ,促进思想政治工作的有效运行。本文分析了形象思维在工作主体思维方式中的地位 ,阐述了在思想政治工作中形象思维的过程、特征及其积极意义与作用     

Balancing a one‐way corridor capacity and safety‐oriented reliability: A stochastic optimization approach for metro train timetabling

In urban rail transit systems of large cities, the headway and following distance of successive trains have been compressed as much as possible to enhance the corridor capacity to satisfy extremely high passenger demand during peak hours. To prevent train collisions and ensure the safety of trains, a safe following distance of trains must be maintained. However, this requirement is subject to a series of complex factors, such as the uncertain train braking performance, train communication delay, and driver reaction time. In this paper, we propose a unified mathematical framework to analyze the safety‐oriented reliability of metro train timetables with different corridor capacities, that is, the train traffic density, and determine the most reliable train timetable for metro lines in an uncertain environment. By employing a space‐time network representation in the formulations, the reliability‐based train timetabling problem is formulated as a nonlinear stochastic programming model, in which we use 0‐1 variables to denote the time‐dependent velocity and position of all involved trains. Several reformulation techniques are developed to obtain an equivalent mixed integer programming model with quadratic constraints (MIQCP) that can be solved to optimality by some commercial solvers. To improve the computational efficiency of the MIQCP model, we develop a dual decomposition solution framework that decomposes the primal problem into several sets of subproblems by dualizing the coupling constraints across different samples. An exact dynamic programming combined with search space reduction strategies is also developed to solve the exact optimal solutions of these subproblems. Two sets of numerical experiments, which involve a relatively small‐scale case and a real‐world instance based on the operation data of the Beijing subway Changping Line are implemented to verify the effectiveness of the proposed approaches.     

从世界主要国家动员实践看我国国防动员应急应战一体化体制建设

本文透过世界主要国家国防动员应急应战的动员实践,认真分析了我国国防动员系统在历次抗灾救灾中所暴露的主要问题,并以此为切入点从应急应战领导指挥体制、应急应战力量体系、物资装备保障体系、应急应战法规体系等四个方面,对构建我国应急应战一体化体制进行了深入的研究探讨,提出了具有一定实践价值的意见建议。     

论东北战场1947年夏季攻势

全国解放战争时期，东北战场最先揭开战略反攻序幕，民主联军从吉林以东、长春以西、郑家屯以北、梅河口以南及辽南、热西、冀东等七个重点方向上同时发动进攻，其势如潮涌，纵横关内外。大战结果，民主联军于战略上贯通南北满，一举扭转东北战局，开始掌握战场主动权，迫使东北国民党军转入战略防御，有力地支援了关内战场作战。     

中世纪外国军事思想初探

军事思想是人们对战争、军队和国防的基本问题的理性认识。研究和总结人类各个时期军事思想的历史发展和特点,是对军事领域问题探讨的过程之一,是人类在军事领域自我认知的手段和途径,是超越自我、实现突破的必经之路。中世纪世界军事思想在特定的历史条件下.经历了一个缓慢的历史演变过程,其战争观、军队建设和战略战术思想有了较大的发展,呈现出缓慢性、演进性和多元性的特征。